ING1b (D3D5Z) Rabbit mAb #14625
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 33 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
ING1b (D3D5Z) Rabbit mAb recognizes endogenous levels of total ING1b protein. This antibody specifically recognizes the ING1b p33 isoform (UniProt# Q9UK53-2).
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ING1b protein.
Background
Inhibitor of growth 1 (ING1) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators (1,2). Differential mRNA splicing generates several ING1 isoforms, with the widely expressed ING1b (p33) isoform playing key roles in cell cycle regulation, apoptosis, and senescence (2-5). All ING family proteins contain a plant homeodomain (PHD) that is thought to recognize and bind methylated lysine residues on histone proteins (6,7). The ING1 protein regulates expression of genes through its association with histone acetyltransferase and deacetylase complexes. The PHD of ING1 may facilitate the recruitment of these chromatin-modifying enzymes to targets genes that are regulated by various transcription factors, such as p53 (2, 8-10). Consistent with its role as a tumor suppressor, alterations in ING1 expression levels and cytoplasm localization have been observed in several cancers but mutations in the corresponding ING1 gene in cancers are uncommon (11-14).
Limited Uses
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