Cat. # | Size | Qty. | Price |
---|---|---|---|
53884S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 105, 150 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:1000 - 1:2000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our #12727 Immunofluorescence Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies:
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 24
Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His747 of human LAMC2 protein.
Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of α (LAMA), β (LAMB), and γ (LAMC) subunits. LAMC2 is the γ subunit of laminin-5 (LN-332 or LN5), which is a triplex composed of laminin α3, β3, and γ2 chains. LN-332 is one of the main isoforms of laminin associated with malignant transformation, proliferation, adhesion, migration, invasion, and metastasis (2). Upregulation of LAMC2 is a prognostic marker for the progression of various cancer types, including colorectal carcinoma, urinary bladder carcinoma, pancreatic ductal adenocarcinoma, and lung carcinoma (3-6). Under physiological conditions, LN-332 locates at the dermal-epidermal junction, provides skin integrity and resistance to external mechanical forces, and promotes adhesion and migration of epithelial cells (7,8). Mutation or deletion of the LAMC2 gene causes Herlitz junctional epidermolysis bullosa, an inherited disease causing severe skin blistering (9,10). The biological activity of LN-332 can be regulated by LAMC2 proteolytic processing through MT1-MMP (MMP14) (11). LN-332 containing full-length LAMC2 (150 kDa) functions to promote adhesion, while LN-332 containing processed LAMC2 (105 kDa) promotes migration (12).
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