Langerin (D9H7R) XP® Rabbit mAb #13650
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:3200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #49830.
Protocol
Specificity / Sensitivity
Langerin (D9H7R) XP® Rabbit mAb recognizes endogenous levels of total Langerin protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human Langerin protein.
Background
Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin including Langerhans cells in the epidermis and langerin+/CD103+ dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8).
- Valladeau, J. et al. (2000) Immunity 12, 71-81.
- Poulin, L.F. et al. (2007) J Exp Med 204, 3119-31.
- Ginhoux, F. et al. (2007) J Exp Med 204, 3133-46.
- Bursch, L.S. et al. (2007) J Exp Med 204, 3147-56.
- Kissenpfennig, A. et al. (2005) Mol Cell Biol 25, 88-99.
- Stambach, N.S. and Taylor, M.E. (2003) Glycobiology 13, 401-10.
- Idoyaga, J. et al. (2008) J Immunol 180, 3647-50.
- Igyártó, B.Z. et al. (2011) Immunity 35, 260-72.
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