Mitochondrial Marker Antibody Sampler Kit #8674
Product Information
Kit Usage Information
Protocols
- 2426: Western Blotting, Immunoprecipitation (Magnetic)
- 3205: Western Blotting, Immunohistochemistry (Paraffin)
- 4266: Western Blotting, Immunoprecipitation (Agarose)
- 4280: Western Blotting, Immunohistochemistry (Paraffin)
- 4661: Western Blotting, Immunohistochemistry (Paraffin)
- 4850: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 7074: Western Blotting
- 7076: Western Blotting
- 11998: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence*
- 12165: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
Specificity / Sensitivity
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein, residues 67-78 of human cytochrome c protein, Trp68 of human HSP60 protein, the sequence of human pyruvate dehydrogenase protein, Gly166 or human SDHA protein, or residues near the amino terminus of human VDAC-1 protein. Mouse monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.
Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1 protein. Polyclonal antibody is purified by protein A and peptide affinity chromatography.
Background
The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).
- Ostermeier, C. et al. (1996) Curr Opin Struct Biol 6, 460-6.
- Schägger, H. (2002) Biochim Biophys Acta 1555, 154-9.
- Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83.
- Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
- Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
- Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
- Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9.
- Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.
Limited Uses
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