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XML generation date: 2024-09-30 01:55:57.063
Product last modified at: 2024-11-15T12:45:15.184Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. N6-Methyladenosine (m6A) (D9D9W) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

N6-Methyladenosine (m6A) (D9D9W) Rabbit mAb #56593

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    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • DB-Dot Blot 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    This antibody has been shown by an independent laboratory to work in RNA-IP-seq. Please use at an assay-dependent dilution.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    N6-Methyladenosine (m6A) (D9D9W) Rabbit mAb recognizes endogenous levels of N6-methyladenosine (m6A). This antibody has been validated using ELISA and dot blot assays and shows high specificity for m6A. This antibody does not cross-react with unmodified adenosine, N6-dimethyladenosine, N1-methyladenosine, or 2'-O-methyladenosine.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with N6-methyladenosine.

    Background

    N6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. While the presence of m6A in RNA was described decades ago, the lack of tools has made interrogating the epitranscriptomic landscape challenging (1,2). With the emergence of new technologies such as miCLIP and NG-RNA-seq, researchers have been able to show that m6A is a biologically relevant mark in mRNA that is enriched in 3’ UTRs and stop codons (3,4). The m6A writer complex consists of a core heterodimer of methyltransferase-like protein 3 (METTL3) and methytransferase-like protein 14 (METTL14), and the additional regulatory proteins Virlizer/VIRMA and Wilms tumor 1-associated protein (WTAP) (5). METTL3 is the catalytic methyltransferase subunit and METTL14 is the target recognition subunit that binds to RNA (6). The Virilzer/VIRMA protein directs m6A methylation to the 3’ UTRs and stop codons, and WTAP targets the complex to nuclear speckles, which are sites of RNA processing (7). Less is known about readers and erasers of m6A, and while the fat mass and obesity-associated protein FTO was the first discovered m6A demethylase, subsequent studies demonstrated that this enzyme may prefer the closely related m6Am mark in vivo (8,9). ALKBH5 was later shown to be a bona fide m6A demethylase enzyme, contributing to the idea that the m6A modification is dynamically regulated (10). Readers of the m6A mark include the YTH protein family, which can bind to m6A and influence mRNA stability and translation efficiency (3,11-13). The m6A mark and machinery have been shown to regulate a variety of cellular functions, including RNA splicing, translational control, pluripotency and cell fate determination, neuronal function, and disease (1, 14-17). The m6A writer complex has been linked to various cancer types including AML and endometrial cancers (18,19). Additionally, m6A has been implicated in resistance to chemotherapy (20).
    1. Meyer, K.D. and Jaffrey, S.R. (2017) Annu Rev Cell Dev Biol 33, 319-42.
    2. Desrosiers, R. et al. (1974) Proc Natl Acad Sci U S A 71, 3971-5.
    3. Dominissini, D. et al. (2012) Nature 485, 201-6.
    4. Meyer, K.D. et al. (2012) Cell 149, 1635-46.
    5. Liu, J. et al. (2014) Nat Chem Biol 10, 93-5.
    6. Wang, X. et al. (2016) Nature 534, 575-8.
    7. Ping, X.L. et al. (2014) Cell Res 24, 177-89.
    8. Jia, G. et al. (2011) Nat Chem Biol 7, 885-7.
    9. Mauer, J. et al. (2017) Nature 541, 371-75.
    10. Zheng, G. et al. (2013) Mol Cell 49, 18-29.
    11. Schwartz, S. et al. (2013) Cell 155, 1409-21.
    12. Wang, X. et al. (2014) Nature 505, 117-20.
    13. Wang, X. et al. (2015) Cell 161, 1388-99.
    14. Batista, P.J. et al. (2014) Cell Stem Cell 15, 707-19.
    15. Batista, P.J. (2017) Genomics Proteomics Bioinformatics 15, 154-63.
    16. Patil, D.P. et al. (2016) Nature 537, 369-73.
    17. Wang, C.X. et al. (2018) PLoS Biol 16, e2004880.
    18. Barbieri, I. et al. (2017) Nature 552, 126-31.
    19. Liu, J. et al. (2018) Nat Cell Biol 20, 1074-83.
    20. Dai, D. et al. (2018) Cell Death Dis 9, 124.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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