NDRG4 (D4A6) Rabbit mAb #9039
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37-45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:800 |
| Immunofluorescence (Frozen) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Unlike other members of the family that are widely expressed, NDRG4/Bdm1 expression is restricted primarily to the heart and brain (2,3,6,7). Multiple alternatively spliced isoforms of NDRG4 have been reported (3,7). Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease (3). In contrast, NDRG4 expression is elevated in glioblastoma and contributes to cell cycle progression and survival (8). NDRG4 also may be inactivated by promotor methylation in colorectal cancer and function as a tumor suppressor gene (9).
- Shimono, A. et al. (1999) Mech Dev 83, 39-52.
- Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
- Zhou, R.H. et al. (2001) Genomics 73, 86-97.
- Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
- Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
- Yamauchi, Y. et al. (1999) Brain Res Mol Brain Res 68, 149-58.
- Nakada, N. et al. (2002) Brain Res Dev Brain Res 135, 45-53.
- Schilling, S.H. et al. (2009) J Biol Chem 284, 25160-9.
- Melotte, V. et al. (2009) J Natl Cancer Inst 101, 916-27.
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