SARS-CoV-2 ORF8 (E6H7V) Rabbit mAb #26856
- WB
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
SARS-CoV-2 ORF8 (E6H7V) Rabbit mAb recognizes endogenous levels of total SARS-CoV-2 ORF8 protein in virus infected cells.
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val62 of SARS-CoV-2 ORF8 protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). ORF8 is transcribed and translated from an ORF in the nested subgenomic RNA of the SARS-CoV-2 genome. It is one of the most divergent proteins in the SARS-CoV genome, and is believed to interfere with the immune response by binding to MHC-1 molecules and targeting them for lysosomal degradation via autophagy (3,4).
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Zhang, Y. et al. (2021) Proc Natl Acad Sci U S A 118, e2024202118. doi: 10.1073/pnas.2024202118.
- Flower, T.G. et al. (2021) Proc Natl Acad Sci U S A 118, e2021785118. doi: 10.1073/pnas.2021785118.
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