Sec31A (E1J5M) Rabbit mAb #13483
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95-140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Protocol
Specificity / Sensitivity
Sec31A (E1J5M) Rabbit mAb recognizes endogenous levels of total Sec31A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1080 of human Sec31A protein.
Background
The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).
- Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
- Miller, E.A. et al. (2003) Cell 114, 497-509.
- Mossessova, E. et al. (2003) Cell 114, 483-95.
- Barlowe, C. et al. (1994) Cell 77, 895-907.
- Bi, X. et al. (2007) Dev Cell 13, 635-45.
- Shugrue, C.A. et al. (1999) J Cell Sci 112 ( Pt 24), 4547-56.
- Tang, B.L. et al. (2000) J Biol Chem 275, 13597-604.
- Stankewich, M.C. et al. (2006) J Cell Sci 119, 958-69.
- Van Roosbroeck, K. et al. (2011) Blood 117, 4056-64.
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