Stargazin (D33G3) Rabbit mAb #8511
- WB
- IP
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Stargazin (D33G3) Rabbit mAb recognizes endogenous levels of total stargazin protein.
Species Reactivity:
Mouse, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human stargazin protein.
Background
Stargazin is a four-pass transmembrane protein related to VDCC (voltage dependent calcium channel) γ subunits and part of the TARP (transmembrane AMPA receptor regulatory protein) family of proteins. TARP proteins can form a complex with AMPA receptors (GluR1-4) and serve as integral auxiliary subunits (1-6).
Interactions between stargazin and AMPA receptors are implicated in regulation of receptor surface expression, synaptic clustering and recycling, as well as increased receptor responsiveness to glutamate (1,2,5,6). Stargazin may play a role in the molecular mechanism of AMPAR-mediated inflammatory pain by taking part in signaling pathways that relay pain in the spinal cord (5). Because the protein also modulates the pharmacology of AMPA receptors, it enhances the effects of AMPAR potentiators that have therapeutic potential for a number of mental and neurodegenerative diseases (6).
The carboxy terminus of the stargazin protein interacts with the PDZ domains of PSD95 and other membrane-associated guanylate kinase (MAGUK) family members, and together traffic AMPA receptors to the cell surface membrane, anchoring them to the postsynaptic site (1,7). Phosphorylation of stargazin by PKA on Thr321 inhibits this binding (3).
Limited Uses
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