Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody #80403
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
Reconstitute with 2.0 ml of dH20 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.
Recommended Antibody Dilutions:
ELISA (chromogenic substrates): 1:5K-1:100K
Western Blotting (chromogenic substrates): 1:5K - 1:100K
Western Blotting (ECL substrates): 1:10K - 1:200K
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Specificity / Sensitivity
Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody detects the heavy chain of human IgA but not human IgG or IgM. This antibody does not detect other non-immunoglobulin serum proteins. It may cross-react with IgA from other species.
Species Reactivity:
Human
Source / Purification
Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody is produced by immunizing goats with human IgA. The Anti-Human IgA, alpha-Chain is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Limited Uses
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