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Render Timestamp: 2024-09-27T09:39:48.412Z
Commit: 53da7bbf777da8a463c431a64421017667e9ff1d
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. RACK1 Antibody

RACK1 Antibody #4716

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Dm Z
    SENSITIVITY Endogenous
    MW (kDa) 32
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Dm-D. melanogaster 
    • Z-Zebrafish 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RACK1 Antibody recognizes endogenous levels of total RACK1 protein.

    Species Reactivity:

    Human, Mouse, Rat, D. melanogaster, Zebrafish

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human RACK1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).

    Database Links

    UniProt ID: P63244


    Entrez-Gene Id: 10399


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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