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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Human Interleukin-1α (hIL-1α)

Human Interleukin-1α (hIL-1α) #5236

This product is discontinued

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    Product Information

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT and 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT.

    Storage

    Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
    Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 18
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.
    Endotoxin Less than 0.01 ng endotoxin/1 μg hIL-1α.
    Activity The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-15 pg/ml.
    Molecular Formula Recombinant hIL-1α does not have a Met on the amino terminus and has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.

    Source / Purification

    Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566) was produced in E. coli at Cell Signaling Technology.

    Background

    IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1α activity is inhibited through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

    Database Links

    UniProt ID: P01583


    Entrez-Gene Id: 3552


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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