Choosing the best ELISA kit for your research is important. Decide if the FastScan, PathScan, or PathScan RP ELISA kit is right for you.
| FastScan™ ELISA Kits | PathScan® ELISA Kits | PathScan® RP ELISA Kits | |
|---|---|---|---|
| Assay type | Solution-based sandwich | Solid-phase sandwich | Solid-phase sandwich |
| Hands-on time | 15 min | 25 min | 15 min |
| Typical time to result | 1.5 hr | 4 hr | 1.5 hr |
| Number of wash steps | 1 | 3 | 1 |
| Sensitivity | Comparable or better than traditional ELISA | Comparable to traditional ELISA | Comparable to traditional ELISA |
| Dynamic Range | Comparable to traditional ELISA | Comparable to traditional ELISA | Comparable to traditional ELISA |
| ELISA Performance Control | Yes | No | No |
| Detection | Colorimetric | Colorimetric (Chemiluminescence is available for some kits.) | Colorimetric (Chemiluminescence is available for some kits.) |
| Plate Format | 96-well – Available 384-well – Inquire |
96-well – Available 384-well – Inquire |
96-well – Available 384-well – Inquire |
Inquire for availability, processing time, and pricing if you are interested in placing bulk orders of five or more kits, 96- to 384-well kit conversions, or colorimetric to chemiluminescent kit conversions.
Generate ELISA data faster compared to traditional PathScan ELISAs, while preserving the same high standards for sensitivity. FastScan ELISA kits also share the same high-speed assay length as PathScan kits optimized for Rapid Protocol (PathScan RP).
Get the highest sensitivity possible for these matched antibody pairs in a traditional assay length and format. PathScan ELISA kits and PathScan kits optimized for Rapid Protocol (PathScan RP) are compatible with the same lysis buffer. This means you can prepare samples in a single buffer and use them across both PathScan and PathScan RP ELISA kits.
PathScan RP ELISAs are optimized with a Rapid Protocol (RP), giving it the same assay time as a FastScan ELISA kit. Unlike the FastScan ELISA kits, RP ELISAs are compatible with the same lysis buffer as a traditional PathScan kit. This means you can prepare samples in a single buffer and use them across both PathScan and PathScan RP ELISA kits.
All three CST ELISA kit product lines are semi-quantitative. They are compatible with various sample types1, including cell extracts and tissue. Kits are available for post-translational modifications, endogenous cellular proteins, and biomarkers across neurodegenerative disease, cancer, and other diseases.
1Other sample types like serum, plasma, and cerebrospinal fluid may be compatible. Samples must be prepared with the included lysis buffer, and originate from a reactive species, as noted on the product page.
CST Scientists develop products along disease signaling pathways so you can move the right candidates along the drug discovery pipeline, faster. They thoroughly validate matched antibody pairs across multiple kit formats to ensure the ELISA kit you receive represents the pinnacle of sensitivity. When sensitivity is equal across multiple formats, they evaluate assay speed. CST ELISA kits are continually optimized to make sure every product you order meets your project needs not just today, or tomorrow, but every day and beyond.
A streamlined format wherein the antibody-target sandwich complex is formed in a single incubation, requiring only one wash. The capture antibody, detection antibody, and experimental sample are combined together before being added to the microplate well. The entire complex is anchored to the well via an anti-tag antibody, which has been pre-adsorbed in the well.
ELISA kits where the capture antibody is pre-coated onto microwell plates. Since the capture antibody is already bound to the plate, the sample, detection antibody, and a secondary HRP-linked antibody can be sequentially applied. Wash steps follow each incubation to ensure unbound material is released, providing a low-background and specific signal.
ELISA kits that also include the capture antibody pre-coated onto microwell plates. Like traditional PathScan ELISA kits, PathScan RP ELISA kits do not require incubation with the capture antibody, since it is already bound to the plate. The key difference is that PathScan RP uses HRP directly conjugated to the detection antibody. Sample, followed by detection antibody, is added to wells with no wash steps in between.
