To achieve high-content imaging (HCI) success, you need to rely on high-quality, trustworthy reagents. CST has high-content analysis (HCA)/high-content screening (HCS) antibodies you can trust to work consistently on your HCI instrument. Use CST® antibodies with confidence to accelerate your screening assays.
Browse IF/High-Content Imaging Antibodies
Many IF-approved CST antibodies, which are ideal for high-content imaging, are available as fully validated fluorescent antibody conjugates. Can’t find the conjugated antibody that you’re looking for? We also offer custom conjugation services to simplify your workload and streamline your project.
CST antibodies are developed with unparalleled validation testing, providing reproducible results for your high-content imaging assay. Expert CST scientists validate every immunofluorescence antibody in-house in multiple ways, including in high-content analysis assays. Learn more about our extensive validation testing.
Our catalog of IF-approved antibodies continues to grow every day. These HCA/HCS-ready antibodies are ideal for examining:
CST antibodies can help you obtain robust fluorescence microscopy images while providing quantitative endpoint analysis for your disease modeling studies. Rest assured, our antibodies will perform optimally as you screen molecular candidates in your high-content analysis or high-content screening assays.
Browse IF/High-Content Imaging Antibodies
All CST antibody products can be purchased in bulk quantities or with antibody lot reservations. To meet your needs, they can also be provided in alternative formulations without BSA or sodium azide. Contact us for more information.
Automated techniques for high-content imaging, like high-content analysis and high-content screening, offer great promise for gaining a deeper understanding of cellular mechanisms in both normal and disease states. These methods can provide an efficient way to evaluate biological processes on multiple levels while providing additional benefits such as increased efficiency and reduced intrinsic user bias.
However, it is crucial to obtain reliable, reproducible, and statistically robust data from high-throughput experiments, so it’s essential to choose your antibodies and reagents carefully. The following videos, posters, and application notes demonstrate how high-content imaging can be used to evaluate biological systems and pathways.
This webinar describes high-throughput methods to investigate TGF-β-induced EMT in biochemical, 2D cellular, and 3D spheroid adenocarcinoma models, using rigorously validated immunoassay reagents from Cell Signaling Technology and the Agilent BioTek Cytation C10 confocal imaging reader.
Find out more about how to optimize your high-throughput, high-content analysis, and high-content screening assays using the following detailed CST Application Notes.
High throughput laser cytometry (heatmap, top) in combination with high content imaging (bottom), are critical tools for all development and production testing of IF-approved antibodies at CST.
CST scientists use HCA for in-house antibody validation.
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Imaged with the confocal imaging mode of the Agilent Cytation C10 (which enables optical resolution of Z-planes in 3D biological samples).
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Images obtained using the Operetta CLS High-Content Analysis System made by Revvity.
