Explore the intricate Wnt Beta Catenin pathway & its role in cell development and disease. Learn more about the signaling cascade of this crucial pathway.
Overview of Adhesion and Extracellular Matrix signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.
List of publications based on primary research done by the Cancer Research Group at CST (2002-2013).
A number of growth factors and cytokines can induce an Epithelial-Mesenchymal Transition (EMT) in tumor cells.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).
A comprehensive list of peer-reviewed publications from Cell Signaling Technology.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Amyloid beta is a protein fragment of the amyloid precursor protein, and is the major component of Alzheimer’s disease plaques.
Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubulin isoforms A, B, and C. The antigen sequences for these antibodies have 96% homology with beta 2C, beta 4B, beta 5B, and beta 4A isoforms 1, 2, and 3, and 95% homology with beta 2C isoforms A and B.
Expert-reviewed signaling pathway providing a current overview of adherens junction dynamics and links to products by Cell Signaling Technology.
Cellular senescence is characterized by irreversible cell-cycle arrest in combination with a distinct secretory phenotype and expanded lysosomes in response to stress. Senescent cells accumulate in tissues during aging, and various markers of senescence
Expert-reviewed diagram providing a current overview of the Mechanisms of Fibrosis pathway with references to its role in adhesion
We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700.
From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may detect other less common isoforms as well, but we have not confirmed this experimentally.
Unfortunately, we are not aware if any of our amyloid-beta antibodies will detect the alpha-oligomer of the amyloid-beta protein.
We believe the 48 kDa band may represent a heterodimer of β-crystallin B1 based on the following references:https://www.ncbi.nlm.nih.gov/gene/1414Dolinska MB, Sergeev YV, Chan MP, Palmer I, Wingfield PT. N-terminal extension of beta B1-crystallin: identification of a critical region that modulates protein interaction with beta A3-crystallin.
We’ve put together a starter’s guide on the cellular mechanisms that drive neurodegeneration in diseases such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis, and Huntington’s disease.
Find Pathways and Diagrams By Disease Area
Our Pan-Calcineurin A Antibody #2614 detects endogenous levels of the alpha isoform of Calcineurin A, however it may also recognize the beta and gamma isoforms. Regrettably, because it has been difficult to find suitable models, we have not specifically tested if this product will detect the beta and gamma isoforms.
Products and Related Resources for Fibrosis SARS-CoV-2 Research
A journal club article elucidating the mechanisms of beta cell failure leading to type 2 diabetes.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
We have several antibodies for β-actin that are validated for IHC-P in human samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700.
A scientific resource for the ADF protein domain containing information on structure, function, and binding to monomeric and filamentous actin.
Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Ser80 in human, which corresponds to Ser79 in mouse and rat.
It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many phosphorylation sites, resulting in a shift in size, or by cross-reactivity with gamma-Actin (beta-Actin = 375 amino acids, gamma-Actin (cytoplasmic form) = 333 amino acids).
Western Blotting troubleshooting guide for easy to solve high and low background issues.
Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: β-Actin (13E5) Rabbit mAb #4970β-Actin (D6A8) Rabbit mAb #8457β-Actin (8H10D10) Mouse mAb #3700β-Actin (E4D9Z) Mouse mAb #58169. All of these antibodies work well and there is no preference for this application.
