DyLight 554 Phalloidin #13054
- IF
Product Information
Product Usage Information
To make a 400X stock, reconstitute the lyophilized material in 750 µl anhydrous DMSO. Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow Cell Signaling Technology protocol for immunofluorescence. Following incubation of the primary and secondary antibodies, DyLight 554 Phalloidin can be diluted 1:400 (0.25 µl per 100 µl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.
Note: Product usage information for this product has changed effective September 01 2024. Previous recommendations were to use either methanol or DMSO as a solvent for lyophilization of the material; It is now recommended to ONLY use DMSO.
Storage
Product Description
DyLight 554 Phalloidin allows researchers to fluorescently label the cytoskeleton of fixed cells through the binding of phalloidin to F-actin. This product is not intended for use on live cells due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 3000 assays based on a 1:400 dilution and a 100 μl assay volume.
DyLight 554 Fluorescent Properties: Excitation: 551 nm, Emission: 572 nm.
Background
Limited Uses
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