TPH2 (D3E5I) XP® Rabbit mAb #51124
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
TPH2 (D3E5I) Rabbit mAb recognizes endogenous levels of total TPH2 protein. Species cross-reactivity for Western blot is human and rat only. Mouse cross-reactivity is determine by immunofluoresence.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu30 of human TPH2 protein.
Background
Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4).
Polymorphisms and regulation of the tph2 gene are involved in cognitive, emotional, and psychiatric disorders (5-7)
Limited Uses
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