CD133 (D2V8Q) XP® Rabbit mAb #64326
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 133 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #51917.
Protocol
Specificity / Sensitivity
CD133 (D2V8Q) XP® Rabbit mAb recognizes endogenous levels of total CD133 protein. This antibody is not sensitive to the glycosylation status of CD133.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312.
Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
- Yin, A.H. et al. (1997) Blood 90, 5002-12.
- Miraglia, S. et al. (1997) Blood 90, 5013-21.
- Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51.
- Monzani, E. et al. (2007) Eur J Cancer 43, 935-46.
- O'Brien, C.A. et al. (2007) Nature 445, 106-10.
- Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5.
- Singh, S.K. et al. (2004) Nature 432, 396-401.
- Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34.
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