SPRY Protein Domain

The SPRY domain was originally discovered as a sequence-repeat in the dual-specificity kinase splA of Dictyostelium and rabbit ryanodine receptor. The ~140 amino acid residue domain adopts a novel fold consisting of a β-sandwich structure formed by two four-stranded antiparallel β-sheets with a unique topology. In combination with the B30.2 domain, these two domains adopt an immunoglobulin-like fold. The SPRY of SSB-2 binds prostate apoptosis response protein-4 (Par-4), while the SPRY domains of RanBPM, RanBP10 and SSB-1 mediate interactions with MET. Mutations found in the SPRY-containing proteins have shown to cause Mediterranean fever and Opitz syndrome.