IMPORTANT: Please refer to the APPLICATIONS section on the front page of the datasheet to determine if this product has been validated and approved for use with this protocol.
NOTE: Prepare solutions with Milli-Q or equivalently purified water.
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
*Recommended Secondary Antibodies:
Anti-Rabbit
Anti-Mouse
Anti-Rat
posted December 2010