Stability Test Results
As a new antibody is developed, Cell Signaling Technology (CST) scientists will typically test the antibody’s stability and activity by western blot after storing samples of the antibody, separately and simultaneously, for a period of 7 days at -20°C, at room temperature, and at 37°C. Antibodies that passed stability testing have been found to be just as active and stable after 7 days of storage at 37°C and 7 days at room temperature as compared to storage at -20°C. To learn more about our stability testing and how you can expect to receive your products from CST, read our blog post on the topic. If you have any further questions, please contact our Technical Support team.
After receiving your order, please continue to store CST antibodies® as indicated on the product sheet.
For all Tests:
A: Antibody was stored at -20°C
B: Antibody was stored at room temperature for one week prior to use.
C: Antibody was stored at 37°C for one week prior to use.
Phospho-p44/42 MAP Kinase (Thr202/Tyr204) Antibody #9101
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-p44/42 MAP Kinase (Thr202/Tyr204) Antibody #9101.
p44/42 MAP Kinase Antibody #9102
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with p44/42 MAP Kinase Antibody #9102.
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251.
SAPK/JNK Antibody #9252
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with SAPK/JNK Antibody #9252.
p38 MAP Kinase Antibody #9212
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with p38 MAP Kinase Antibody #9212.
Phospho-c-Jun (Ser63) II Antibody #9261
Western blot analysis of extracts from HEK293 cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-c-Jun (Ser63) II Antibody #9261.
Phospho-SEK1/MKK4 (Thr261) Antibody #9151
Western blot analysis of extracts from 3T3 cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-SEK1/MKK4 (Thr261) Antibody #9151.
Phospho-HSP27 (Ser82) Antibody #2401
Western blot analysis of extracts from COS cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-HSP27 (Ser82) Antibody #2401.
Phospho-MAPKAPK-2 (Thr334) Antibody #3041
Western blot analysis of extracts from 3T3 cells, untreated or treated with UV light (40mJ/cm2), probed with Phospho-MAPKAPK-2 (Thr334) Antibody #3041.
Anti-rabbit IgG, HRP-linked Antibody #7074
Western blot analysis of extracts from SK-MEL-28 cells probed with EEA1 Antibody #2411 and GAPDH (D16H11) XP® Rabbit mAb #5174. The secondary, Anti-rabbit IgG, HRP-linked Antibody #7074, was incubated at -20, RT, or 37 degrees for 1 week before being used for detection.
If you have any questions or concerns, please contact our Technical Support team.