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Poster Presentations

Scientists from Cell Signaling Technology regularly present posters at scientific conferences. Our studies involve the development of new technologies for signaling pathway analysis and mechanistic cell biology research.

Portable Document Format (PDF) files of our investigative research are linked below. Some of the PDFs are large files. The sizes for each file are listed.

Quantitative evaluation of biomarkers for TGF-β-induced epithelial mesenchymal transition in biochemical, cellular, and 3D spheroid model systems
Ernest Heimsath, Antony Wood
1.0 MB PDF
Development of a High-Plex Antibody Panel for Spatial Phenotyping of FFPE Murine Tissues
Nadezhda Nikulina, Bassem Ben Cheikh, Jeremy Fisher, Christopher Grange, Brooke Ekborg, Jean Loebelenz,Samuel Jensen, Paulina Leung-Lee, Niyati Jhaveri , Tsz H. Tam, Oliver Braubach
4.4 MB PDF
A novel monoclonal antibody for the detection, enrichment and activation of cells expressing scFv-based chimeric antigen receptors
Amrik Singh, Sarah L’Heureux, Ryan Sinapius, Jeremy Fisher
2.2 MB PDF
Spatial resolution of immune cell lineages in the tumor microenvironment of plasma cell dyscrasias
Medbh A. Dillon, Lisa Arvidson, Cole G. Phalen, Ruoxin Li, Imran McGrath, Julian R. Ishibashi, John B. Johanneson, Kevin J. Li, Zachary J.Thomson, Samuel Jensen, Jocelin Malone, Mackenzie S. Kopp, Susan A. Lundmann, Adam K. Savage, Claire E. Gustafson, Marla Glass, Emma L. Kuan, Lucas T. Graybuck, Xiao-jun Li, Troy R. Torgerson, Peter J. Skene, Stephanie Añover-Sombke, Melinda L. Angus-Hill
5.9 MB PDF
Mapping the cancer immune landscape in colorectal adenocarcinoma using multiplexed imaging and AI based analysis
Vasundhara Agrawal , Lisa Arvidson, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Samuel Jensen, Arindam Bose
4.1 MB PDF
Multiplexed analysis across cancers of diverse origins to unravel spatial interactions within the tumor microenvironment
Vasundhara Agrawal , Lisa Arvidson, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Samuel Jensen, Arindam Bose
2.3 MB PDF
Spatial architecture of tumor and immune cell lineages in syngeneic mouse tumor tissues
Arindam Bose, Lisa Arvidson, Emily Quann Alonzo, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Gabriella Spang, Matthew Norton, Samuel Jensen, Vasundhara Agrawal
4.5 MB PDF
Development of an Ultrahigh-plex Antibody Panel for Spatial Phenotyping of Murine Cancer Models
Nadezhda Nikulina, Bassem Ben Cheikh, Jeremy Fisher, Christopher Grange, Brooke, Ekborg, Jean Loebelenz, Samuel Jensen, Paulina Leung-Lee, Oliver Braubach
4.1 MB PDF
Signaling flow cytometry: One-step staining for phenotyping and functional characterization of immune cells
Presented by: Emily Alonzo, PhD, Director of Antibody Discovery, Immunology and Immuno-Oncology at CST and Chris Manning, MS, Associate Director of Flow Cytometry at CST
2.0 MB PDF
A Systematic Comparison of Common Multiplex Immunohistochemistry (mIHC) Methodologies to the Novel SignalStar™ Assay in the Characterization of the Tumor Microenvironment (TME)
J. Ziello, D. Papalegis, L. Vu, K. Goldman, J. Loebelenz, Shelby Sponaugle, S. Tkachev, S. Klein
3.1 MB PDF
A Novel Monoclonal Antibody for the Detection, Enrichment and Activation of Cells Expressing scFv-Based Chimeric Antigen Receptors
A. Singh, S. L'Heureux, R. Sinapius, J. Fisher
2.1 MB PDF
Selecting Your Epigenomic Assay: Comparing ChIP-seq, CUT&RUN, and CUT&Tag
A. H. Guo, C. R. Comeau, F. Chen
0.9 MB PDF
Characterization of the Autophagic-Lysosomal Pathway in Parkinson's Disease Using Patient iPSC-Derived Dopaminergic Neurons Containing Lrrk2 or Gba Mutations
S. Coveney, K. LeFrancois, V. E. Bain, M. Zoller, S. Singh, A. Fathi, S.Schachtele, R. W. Cho
16.3 MB PDF
Spatial Proteomic Analysis of Alzheimer's Disease Human Brain Using Multiplexed Imaging
Arindam Bose, Lisa Arvidson, Richard W. Cho, Supriya Singh, Gabriella Spang, Michael J. Smith, Vasundhara Agrawal
2.5 MB PDF
Development and Characterization of a Novel Monoclonal Antibody to Tau p217 in Alzheimer's Disease Murine Models and Human Disease Tissue
Arica Aiello, Daniel E. Mulhern, Fong Lin, Mark Stump, Supriya Singh, Thorsten Wiederhold, Richard W. Cho
1.3 MB PDF
Improved immunoaffinity enrichment methods for arginine methylation
Barry Zee, Hayley Peckham, Charles Farnsworth, Alissa Nelson, Jian Min Ren, Michael Palazzola, Kathryn Abell, Matthew Stokes
1.7 MB PDF
SignalStarTM multiplex immunohistochemistry is a novel technology capable of amplifying the signal of multiple biomarkers in the tumor microenvironment
Jennifer Ziello, Derek Papalegis, Lily Vu, Sasha Tkachev, Sarah Klein
8.5 MB PDF
SignalStarTM multiplex immunohistochemistry is a flexible spatial technology with fully validated protocols
Derek Papalegis, Lily Vu, Sasha Tkachev, Jean Loebelenz, Jeremy Fisher, Keith Arlotta, Sarah Klein
13 MB PDF
Generation and validation of anti-linker monoclonal antibodies for the detection of surface expressed scFv-based CARs
Amrik Singh, Sarah L'Heureux, Ryan Sinapius, Michael Kvorjak, Emma Wade, Jason Lohmueller, Jeremy Fisher, Reginaldo Prioli
1.9 MB PDF
Accelerating eCLIP Studies - Importance of Antibody Validation and Pre-Validated Antibodies for RNA Binding Proteins
Brian Lee, Steven Blue, Curtis Desilets, Carrie A. Brown, Daniel Lorenz, Jake Solorzano, Stephanie C. Bruns, Marco Branchette, Chtistopher J. Fry, Gene W.Yeo
15.5 MB PDF
Analyses of epigenetic marks and mechanisms in disease: Your guide to a successful CUT&RUN assay
Angela H. Guo, Christopher R. Comeau, Fang Chen, Christopher J. Fry
11.4 MB PDF
A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway
Ernest Heimsath, Antony Wood, Lisa Arvidson, and Reginaldo Prioli
849 KB PDF
Reproducibility in spatial biology Reducing variables to improve the reliability of insight generation
Lisa Arvidson, Reginaldo Prioli, Samuel Jensen, James B. Hoying, Michael W. Golway, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Hideki Sasaki, Chi-Chou Huang, Tuan H. Phan, Melinda L. Angus-Hill
24 MB PDF
Spatial resolution of the tumor and immune cell lineages in the hypoxic microenvironment of pancreatic ductal adenocarcinoma
Lisa Arvidson, Reginaldo Prioli, Samuel Jensen, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Hideki Sasaki, Chi-Chou Huang, Tuan H. Phan, Melinda L. Angus-Hill
25.2 MB PDF
Development of control peptides for immunoaffinity-based enrichment of posttranslationally modified peptides
Barry M. Zee, Hayley J. Peckham, Charles L. Farnsworth, Alissa J. Nelson, Kathryn E. Abell, Vicky Yang, Jeffrey Knott, Matthew P. Stokes
12.6 MB PDF
Capturing the spatial landscape of tumor and immune cell lineages in the microenvironment of human cancer tissues.
Reginaldo Prioli, Lisa Arvidson, Sam Jensen, Michael J. Smith, Katie O. White, Richard A. Heil-Chapdelaine, Hideki Sasaki, Chi-Chou Huang, Tuan H. Phan, Melinda L. Angus-Hill
5.40 MB PDF
Improved immunoaffinity enrichment methods for tyrosine phosphorylated, lysine acetylated, and lysine succinylated peptides with high sensitivity and specificity.
Barry M. Zee, Hayley J. Peckham, Alissa J. Nelson, Charles L. Farnsworth; Kathryn E. Abell, Jian Min Ren, Michael C. Palazzola, Kelly D. Peterson, Matthew P. Stokes
3.66 MB PDF
Development of novel rabbit monoclonal antibodies to characterize microglial activation states in murine models of Alzheimer’s disease.
Arica Aiello, Rebecca Gray, Virginia E. Bain, Amrik Singh, Gregory Innocenti, Supriya Singh, Thorsten Wiederhold, Richard W. Cho
25.6 MB PDF
Development of novel TREM2 rabbit monoclonal antibodies that activate downstream, Syk-associated cell signaling pathways.
Jordan W. Hirschfeld, Kamilla Leao, Adriana LaMarca, Sara Roberts, Thorsten Wiederhold, Richard W. Cho
14.8 MB PDF
Phosphoproteomics reveals GITR agonism differentially regulates inflammatory vs. regulatory T cell subsets
Andrew Davis, Lori Cunningham, Matthew Stokes, Alissa Nelson, Kathryn Abell, Michael Lewis, Roy Scialdone, Than Bui, Emily Alonzo, Gordon Freeman, Sarah R Klein
3.55 MB PDF
An Immunohistochemical Tool to Visualize Hydroxyproline in FFPE Tissue
Antony Wood, Lauren Carr, Jessica Simendinger, Laura Vehring, Susan Kane, Katherine Crosby
56.2 MB PDF
Analysis of Epigenetic Marks and Mechanisms in Disease: Your Guide to a Successful CUT&RUN Assay
Fang Chen, Christopher R. Comeau, Jillian Mason, Carrie A. Brown, and Christopher J. Fry
17.43 MB PDF
Development and Characterization of Antibodies Specific for the SS18-SSX Fusion Protein in Synovial Sarcoma
Curtis Desilets, Fang Chen, Christopher Comeau, Katrina Costa, Katherine Crosby, Michelle Ferrante, Christopher Grange, Maggie Patrikas, Matthew McBride, Esther Baranov, Jason Hornick, Cigall Kadoch
15.8 MB PDF
Visualization of Global Collagen in FFPE Tissues Using a Hydroxyproline Antibody
Lauren Carr, Jessica Simendinger, Laura Vehring, Susan Kane, Katherine Crosby
2.86 MB PDF
Improved Immuno-affinity Enrichment Method for Ubiquitinated Peptides with High Sensitivity, Specificity and Robustness
Joshua Nathan, Yiying Zhu, Alissa J. Nelson, Michael C. Palazzola, Kathryn E. Abell, Charles L. Farnsworth, Jian Min Ren, Matthew P. Stokes, Kimberly A. Lee
3 MB PDF
SignalScan Targeted LCMS/MS Assay to Profile Changes in Protein and Phosphorylation Levels with SARS-CoV-2 Infection and Treatment
Yiying Zhu, Alissa J. Nelson, Amrik Singh, Susan Kane, Hayley Peckham, Jian Min Ren, Claire Simpson, Joshua Nathan, Tyler Levy, Tomer M. Yaron, Jared Johnson, Robert Schwartz, Benjamin Nilsson-Payant, Tristan Jordan, Benjamin R. tenOever, Lewis C. Cantley, Sean A. Beausoleil, Kimberly A. Lee
3 MB PDF
Investigating the Hallmarks of Cancer: The importance of rigorous reagent validation for research reproducibility
Antony W. Wood, Susan Keezer, Gary Kasof, Jing Li, Jianxin Xie, Thorsten Wiederhold, Joshua Nathan and Samuel Jensen
6.22 MB PDF
Quantitation of Peptides by Miniature Mass Spectrometry
Spencer Chiang, Wenpeng Zhang, Kimberly Lee, Zheng Ouyang
4.13 MB PDF
Identification and Quantitation of Phosphopeptide Positional Isomers using Trapped Ion Mobility Spectrometry and PASEF
Christopher M. Adams, Michael Krawitzky, Katherine Tran, Baozhen Shan, Zac Anderson, Charles Farsnsworth, Matthew P. Stokes, Kimberly Lee, Shourjo Ghose, Matthew Willetts, Gary Kruppa
0.43 MB PDF
High Sensitivity PTM Characterization in Complex Cell Lysates Using Trapped Ion Mobility
Matthew Willetts, Shourjo Ghose, Gary Kruppa, Matthew Stokes, Charles Farnsworth and Kimberly Lee
0.26 MB PDF
Improved reproducibility of enrichment and site-assignment of biotinylated peptides using new anti-biotin antibody and its application to investigate redox signaling
Meagan E. Olive, Yiying Zhu, Kimberly A. Lee, Samuel A. Myers, Namrata D. Udeshi, and Steven A. Carr
0.97 MB PDF
Automated PTMScan® immunoaffinity enrichment for the capture of KGG modified peptides from complex mixtures
Lilian Phu, Shadie Nimri, Anne Baldwin, Nadia Martinez Martin, Chris Suh, Matthew P. Stokes and Donald S. Kirkpatrick
11.56 MB PDF
ABRF-sPRG 2018-2019: Development and Characterization of a Stable-Isotope Labeled Phosphopeptide Standard
Anthony Herren, Brian Searle, Ryan Leib, Kimberly Lee, Bhavin Patel, David Hawke, Paul Stemmer, Alexandre Rosa Campos, Allis Chien, Antonius Koller
1.03 MB PDF
ABRF-sPRG 2017-2018: Development and Characterization of a Stable-Isotope Labeled Phosphopeptide Standard
Anthony Herren, Brian Searle, Ryan Leib, Kimberly Lee, Bhavin Patel, David Hawke, Brett Phinney, Allis Chien, Antonius Koller
0.83 MB PDF
Bead Assisted Mass Spectrometry(BAMS):A Robust Affinity Capture, MS Method for Multiplexed Biomarker Profiling
Yiying Zhu, Matthew D. Fry, Alissa J. Nelson, Jianmin Ren, Vicky Yang, Michael C. Palazzola, Charles L. Farnsworth, Matthew P. Stokes, Kimberly A. Lee
1.46 MB PDF
Novel Rabbit Monoclonal Antibodies for Profiling of Ser/Thr O-GlcNAc Modified Proteins
Matthew D. Fry, Rami Najjar, Yiying Zhu, Jian Min Ren, Devin K. Schweppe, Steven P. Gygi, Matthew P. Stokes, Kimberly A. Lee
0.72 MB PDF
Quantitative Profiling of Post-Translational Modifications in E. coli
Charles L Farnsworth, Xiaoying Jia, Jian Min Ren, Kimberly Lee, Justin Tropp, Wayne Gatlin, Zach Norring
1.6 MB PDF
Novel antibody reagents for the characterization of Protein ADP-ribosylation
Alvin Lu, Rami Najjar, Mario Niepel, Matthew P Stokes
2.9 MB PDF
PD-1 blockade activates conventional CD4+ T cells and the innate immune response during glioblastoma eradication
Sarah Klein, Jennifer Ziello, Maria Speranza, Prafulla Gokhale, Paul Kirschmeier, Katherine Crosby, Gordon Freeman, David Reardon
2.2 MB PDF
Characterization of Immune Infiltrate and Checkpoint Protein Expression Patterns in Murine Syngeneic Tumors via Multiplex Immunohistochemistry
Jennifer Ziello, Sarah Klein, Katrina Costa, Katherine Crosby
1.3 MB PDF
Mass spectrometry-based profiling of lysine acetylation and arginine methylation for biomarker discovery in triple negative breast cancer
Alissa J. Schunter, Hongbo Gu, Jian Min Ren, Vicky Yang, Matthew P. Stokes
1.1 MB PDF
Multiplexed cellular signaling pathway profiling through targeted peptide immunoenrichment and quantitative analysis by LCMS
Kimberly A. Lee, Yiying Zhu, Jian Min Ren, Charles L. Farnsworth, Matthew P. Stokes
1.5 MB PDF
Co-expression and Frequency of Immune Checkpoint Proteins in the Tumor Microenvironment Analyzed via Multiplex Immunohistochemistry
Jennifer Ziello, Sarah Klein, Katherine Crosby
1.9 MB PDF
Development of a glycosylation-independent, IHC-validated recombinant Rabbit monoclonal antibody against the cancer stem cell marker CD133
Antony W Wood, Richard Cotta, Matthew Prokop, Jessica Simendinger
3.4 MB PDF
Multiplex IHC Detection of Immune Checkpoint Receptors in the Tumor Microenvironment
Jennifer Ziello, Sarah Klein, Emily Alonzo, Herbert Haack, Katherine Crosby
2.8 MB PDF
Targeting critical signaling nodes using multiplexed antibody based phosphopeptide enrichment with iMRM validation
Charles L Farnsworth, Yiying Zhu, Xiaoying Jia, Kimberly A Lee, Matthew P Stokes
2.2 MB PDF
Highly Multiplexed IHC Assays to Examine Immune Checkpoints and Biomarkers for Immunotherapy
Jennifer Ziello, Sarah Klein, Lauren Carr, Katherine Crosby, Emily Alonzo, Herbert Haack
8.3 MB PDF
The Use of Highly Validated Recombinant Monoclonal Antibodies to Analyze Histone Modifications and Transcription Factor Binding by ChIP-seq
Fang Chen, Yuichi Nishi, Dennis J. O'Rourke, Curtis D. Desilets, Christopher J. Fry
5.6 MB PDF
Efficient Enrichment of Endogenous SUMOylated Peptides from Alpha-lytic Protease Digest Using K-Ɛ-GG Remnant Immuno-affinity Purification
Hongbo Gu, Xiaoying Jia, Jianmin Ren, Elizabeth Komives, Matthew Stokes
3 MB PDF
Quantitative Profiling of Prokaryotic Post Translational Modifications
Charles L. Farnsworth, Xiaoying Jia, Jian Min Ren, Kimberly Lee, Jake Namaroff, Matthew P. Stokes
1.8 MB PDF
New Antibody Reagents to Study Stem Cell-Mediated Development and Regeneration of the Mammalian Intestinal Epithelium
Antony W. Wood, Richard Cotta, Katherine Crosby, Aparna Viswanathan, Christine Gagan, Jessica Simendinger
1.3 MB PDF
Global, Site Specific Identification and Quantification of Endogenous SUMO Modifications under Native Conditions
Hongbo Gu, Xiaoying Jia, Jian Min Ren, Elizabeth Komoves, Matthew P. Stokes
1.3 MB PDF
Sequential Immunoaffinity Purification of Post-Translationally Modified Peptides for Improved Enrichment Specificity
Matthew P. Stokes, Charles L. Farnsworth, Hongbo Gu, Xiaoying Jia, Kimberly A. Lee, Jian Min Ren, Vicky Yang
1.5 MB PDF
Development and Validation of a Novel EGF Receptor-Neutralizing (D1D4J) Monoclonal Antibody
Krystyna Zuberek Hincman, Christopher A. Manning, Michael Nelson, Michael Lewis, Roy Scialdone, Jaime Darce, Stephen Lutz, Evans John Burford, Herbert Haack, Matthew Silver
0.8 MB PDF
Proteomic Analysis Identifies Multi-Dimensional Deregulated Signaling Pathways in SCLC Lung Cancer
Klarisa Rikova, Ben Hall, Tyler Levy, Anthony Possemato, Mike Aguiar, Sean Beausoleil, Jian Min Ren, Kimberly Lee, Scott Lonning, Michael Comb
0.8 MB PDF
The Use of Highly Validated Rabbit Monoclonal Antibodies to Analyze Epigenetic Marks and Mechanisms in Disease
Christopher J. Fry, Curtis D. Desilets, Mieke Sprangers, Sarah L. Lambert, Lana Peckham, Jillian Mason, Dennis J. O’Rourke, Yuichi Nishi, Sayura Aoyagi.
3.9 MB PDF
Demonstration of Orthogonal Complementary Enrichment Methods for Enhanced Phosphopeptide Profiling of Drug-Treated Gastric Carcinoma Cells
Charles L. Farnsworth, Hongbo Gu, Xiaoying Jia, Jian Min Ren, Kimberly Lee, Jeffrey C. Silva.
7.6 MB PDF
An Improved Immunoaffinity Reagent for Quantitative Profiling of Lysine Acetylation
Jeffrey C. Silva, Ailan Guo, Anthony Couvillon, Rami Najjar, Daniel Mulhern, Kimberly A. Lee, Jian-min Ren, Xiaoying Jia, Hongbo Gu.
2.7 MB PDF
Profiling and Cross-Talk of Tyrosine Phosphorylation with Lysine Acetylation and Succinylation in Tyrosine Kinase Inhibitor Resistant Breast Cancer Cells
Hongbo Gu, Erik J. Soderblom, J. Will Thompson, Wenle Xia, Kimberly A. Lee, Jian-min Ren, Neil Spector, M. Arthur Moseley, Jeffrey C. Silva.
2.8 MB PDF
Automated Immunoaffinity-based Proteomic Methods for the Study of Post-translational Modification
Matthew P. Stokes, Jeffrey C. Silva, Steven Murphy, Jason Russell, Xiaoying Jia, Jian Min Ren, Kimberly Lee.
3.9 MB PDF
Epigenetic Changes Correlate with Loss of Pluripotency Marker Expression During Retinoic Acid-induced Differentiation of NTERA-2 Cells
Antony W. Wood, Susan Kane, Christopher J. Fry, Raffi Manoukian, Curtis D. Desilets
1.4 MB PDF
Highly Parallel Planar Surface Antibody Array-based Assay to Monitor Protein-protein Interactions
Jacob Knowles, Stephen Capodilupo, Richard Noring, Roberto Campos-González, Julio E. Herrera, Zev Gechtman
2.8 MB PDF
Mapping deregulated pathways downstream of RTKs in cell line models.
Klarisa Rikova, Benjamin Hall, Anthony Possemato, Keri Mroszczyk, Kimberly A. Lee, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Michael J. Comb
2.8 MB PDF
Understanding of Differing Sensitivity in EML4-ALK NSCLC Patients to Crizotinib and Geldanamycin.
Klarisa Rikova, Benjamin Hall, Anthony Possemato, Keri Mroszczyk, Kimberly A. Lee, Joan MacNeill, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Michael J. Comb
3.9 MB PDF
Proteomic Based Analysis of Ovarian Cancer Pathways.
Klarisa Rikova, Benjamin Hall, Tyler Levy, Caroline Keroack, Anthony Possemato, Kimberly A. Lee, Joan MacNeill, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Scott Lonning, Michael J. Comb
1.2 MB PDF
Probing EGFR, HER2, and c-Met Protein-Protein Interactions Using an Antibody Array.
Jacob Knowles, Stephen Capodilupo, Richard Noring, Roberto Campos-González, Julio Herrera and Zev Gechtman
2.1 MB PDF
Motif Antibody Enrichment Enables the Identification of a Large Discrete and Complementary Set of Phosphorylation Sites.
Anthony Possemato; Mike Aguiar, Kim Lee, Steven P. Gygi, Sean A. Beausoleil
3.5 MB PDF
Quantitative Profiling of the Acetylome in Mouse Tissue using Lysine Acetylation Reagents in Immunoaffinity LC-MS/MS.
Hongbo Gu, Matthew P. Stokes, Ailan Guo, Kimberly Lee, Jian Min Ren, Xiaoying Jia, Vipin Suri, Jeffrey Silva
3.4 MB PDF
Network and Pathway Analysis of Post-translationally Modified Peptides Identified by Immunoaffinity-based Proteomics.
Jeffrey C. Silva, Stuart Tugendreich, Hongbo Gu, Charles L. Farnsworth, Kimberly A. Lee, Xiaoying Jia, Jian Min Ren, Matthew P. Stokes
6.9 MB PDF
Development and application of highly sensitive SIM and SRM-based phosphopeptide immunoassays using the Q Exactive™ mass spectrometer.
Kimberly A. Lee, Joan MacNeill, Jing Zhou, Jian Yu, Matthew P. Stokes, Jeffrey C. Silva, Ailan Guo, Michael J. Comb
4.4 MB PDF
Quantitative profiling of signaling pathways using immunoaffinity purification and LC-MS/MS.
Matthew P. Stokes, Jian Min Ren, Kimberly A. Lee, Xiaoying Jia, Jeffrey C. Silva
20.5 MB PDF
Quantitative Profiling of the Acetylome in Mouse Tissue using Lysine Acetylation Reagents in Immunoaffinity LC-MS/MS.
Bryon F. Ricketts, Hongbo Gu, Matthew P. Stokes, Ailan Guo, Kimberly Lee, Jian Min Ren, Xiaoying Jia, Vipin Suri, Jeffrey Silva
4.2 MB PDF
Monitoring the evolution of antigen-specific antibodies in circulation and their corresponding B cell clones in longitudinal immunization case studies through the use of NG-XMT™.
Jaime R. Darce, Stephen R. Lutz, Shuji Sato, Jason G. Beaudet, Michael Palazzola, Ivan Gregoretti, Sasha Tkachev, Sean A. Beausoleil, Lana Popova, Ravi K. Ramenani, Elizabeth Moreno, Xiaowu Zhang, James S. Wieler, Sandra M. Schieferl, Roberto D. Polakiewicz, and Wan Cheung Cheung
2.9 MB PDF
Highly Parallel Planar Surface Antibody Array-based Assay to Monitor Protein-protein Interactions.
Jacob Knowles, Stephen Capodilupo, Richard Noring, Roberto Campos-González, Julio E. Herrera, and Zev Gechtman
1.2 MB PDF
Proteomic Characterization of the Evolution of the Circulating Antibody Response to Hepatitis B Virus Vaccination.
Shuji Sato,, Sean A. Beausoleil,, Lana Popova, Jason G. Beaudet, Ravi K. Ramenani, Xiaowu Zhang, James S. Wieler, Albrecht Moritz, Sandra M. Schieferl, Wan Cheung Cheung, and Roberto D. Polakiewicz
3.4 MB PDF
Comprehensive Quantitative Profiling of the Mouse Kinome using Data-independent LC-MS-MS/MSALL with SWATH™ Acquisition.
Jeffrey C. Silva, Matthew P. Stokes, Charles L. Farnsworth, Xiaoying Jia, Christie Hunter
909 KB PDF
Quantitative Profiling of Signaling Pathways Using PTMScan® Direct.
Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Roberto Polakiewicz, Michael J Comb
2.2 MB PDF
The Large-scale Analysis of Protein Expression in Human Ovarian Tumor Samples by LC-MS/MS/MS.
Anthony Possemato, Sean Beausoleil, Klarisa Rikova, Hong Ren, Kimberly Lee, Michael Comb
1.7 MB PDF
Multiplexing Kinase Substrate Motif Antibodies for Expanded Coverage of the Serine/Threonine Kinome.
Charles L. Farnsworth, Jeffrey C. Silva, Matthew P. Stokes, Hongbo Gu, Ailan Guo, Kimberly A. Lee, Jian-Min Ren, Xiaoying Jia, Roberto D. Polakiewicz, Michael J. Comb
717 KB PDF
Immunoaffinity Enrichment and Quantitative Profiling of Protein Phosphorylation, Methylation, and Acetylation in Receptor Tyrosine Kinase Pathway-addicted Cancer Cell Lines.
Klarisa Rikova, Ailan Guo, Kimberly A. Lee, Anthony Possemato, Yi Wang, Sean A. Beausoleil, Daniel E. Mulhern, Jian Min Ren, Michael J. Comb
2.5 MB PDF
A Proteomics Approach for the Identification and Cloning of Monoclonal Antibodies from Serum.
Wan Cheung (Gabriel) Cheung*, Sean A. Beausoleil*, Xiaowu Zhang, Shuji Sato, Sandra M. Schieferl, James S. Wieler, Jason G. Beaudet, Ravi K. Ramenani, Lana Popova, Michael J. Comb, John Rush, and Roberto D. Polakiewicz.
*These authors contributed equally to this work
1.4 MB PDF
Over-expression of EphB1 leads to Stat3 phosphorylation.
Danielle Swain, Richard Noring, Jake Knowles, Roberto Campos-González, Zev Gechtman
1.1 MB PDF
Rapid quantification of autophagy in cell-based assays.
Christopher A. Manning, Michael R. Nelson, Paul Wylie, Diana Caracino, Randall K. Wetzel
1.9 MB PDF
The use of a four-color multiplex antibody-based assay with laser-scanning high content screening to examine kinase inhibitor effects on cellular signaling.
Jessica A. Cherry, Paul Wylie, Christopher A. Manning, Diana Caracino, Randall K. Wetzel
596 KB PDF
PTMScan® Direct: Quantitative Profiling of Critical Nodes From Cellular Signaling Pathways.
Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Albrecht Moritz, Ailan Guo, John Rush, Roberto Polakiewicz, Michael J. Comb
8.1 MB PDF
Mutation-Specific Antibodies for the Detection of EGFR Mutations in Non-Small-Cell Lung Cancer.
Jian Yu, Susan Kane, Daiqiang Li, Herbert Haack, Bradley Smith, Ting-Lei Gu, Massimo Loda, Xinmin Zhou, Michael J. Comb
1.6 MB PDF
Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening.
Jessica A. Cherry, Corinne Michaud, Paul Wylie, Randall K. Wetzel
852 KB PDF
Evaluation of PTEN: Immunohistochemical Analysis of Tissues and Cells.
Angela Lightbown, Qingfu Zeng, Michael Lewis, Katherine Crosby, Bradley L. Smith
360 KB PDF
Detection of Genetic Abnormalities in Cancer Cells using Bio-Plex® Immuno-Assays.
Andrew Romano, Kathleen Rogers, Susan Kane, Jeff Lee, Whei Feng, Bradley L. Smith, Reggie Prioli
246 KB PDF
Phosphorylation Signatures Identify Tyrosine Kinases Activated in Lung Cancer.
Klarisa Rikova, Ailan Guo, Qingfu Zeng, Anthony Possemato, Herbert Haack, Julie Nardone, Kimberly Lee, Yu Li, Laura Sullivan, Joan MacNeill, Corey Bakalarski, Judit Villen, John Kornhauser, John Rush, Steven Gygi, Roberto Polakiewicz, Michael Comb
822 KB PDF
Rapid and Quantitative Cell-Based Immunoaffinity Kinase Assay in a 96-well Format.
Jin Yuan, Craig Thompson
569 KB PDF
The Detection of Phosphoproteins by Bio-Plex® Assays in Cell, Xenograft and Human Tumor Lysate.
Jeremy Fisher, Kathleen Rogers, Katherine Crosby, Andrew Romano, Bradley L. Smith, Reginaldo Prioli
512 KB PDF
PhosphoSite®: A Knowledge Base of Post-Translational Modifications and Their Role in Cell Signaling.
David Merberg, Roland Carel, Jon Kornhauser, Juan Munoz, Beth Murray, Julie Nardone, Ousama Shamma, Elzbieta Skrzypek, Bin Zhang, Peter Hornbeck
1.1 MB PDF
Phospho-Specific Rabbit Monoclonal Antibodies Validated for Immunohistochemistry.
Cynthia Shapiro, Angela Lightbown, Kathleen Rogers, Jessica Simendinger, Michael Lewis, Katherine Crosby
2.9 MB PDF