Render Target: SSR
Render Timestamp: 2025-01-21T03:13:41.509Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
< Back to Support Article Search Results

Can I quantify my samples using the Senescence β-Galactosidase Staining Kit #9860?

The Senescence β-Galactosidase Staining Kit #9860 is intended to be a qualitative assay to determine if cells are senescent. We have not determined a means of quantification in-house. However, we usually direct customers to the following paper where Hoechst (#4082) was used as a DNA dye to quantify cells in frozen tissue: 

Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-9 (PMID: 15208629; https://pubmed.ncbi.nlm.nih.gov/15208629/

The paper below is another we frequently send to customers interested in quantifying their samples: 

Gary, R.K. and Kindell, S.M. (2005) Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts. Anal Biochem 343(2), 329-34 (PMID: 16004951; https://pubmed.ncbi.nlm.nih.gov/16004951/)
 

Last updated: September 12, 2024

Was this article helpful?

Technical Support

Email: support@cellsignal.com

Send Us a Message

Call: 877-678-8324

Customer Support

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355

Fax: 866-432-6112

Contact Sales

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355