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Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

Which phospho-AMPKα antibody do you suggest for my western blot experiment?

The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:

  • Phospho-AMPKα (Thr172) (40H9) R...

    How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

    Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

    What is the solution formulation of the PTMScan Control peptides?

    The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).
     

    Can I combine antibodies used in a SignalStar™ assay with direct conjugates?

    SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit 

    What controls should I include when performing a Simple Western™ experiment?

    In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.

    1. A no lysate control. This includes the primary antibody at t...

    My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

    Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

    Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?

    During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...

    Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

    Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...

    What is the intended use of the PTMScan® Control peptides?

    These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTMScan success and rep...

    I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?

    SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...

    Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?

    No, the use of Fc block is not required when using F(ab') or F(ab')2 antibody fragments in live cell flow cytometry assays, as removal of the Fc domain eliminates the interaction between...

    What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?

    When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...

    What isoforms does the ZBP1 Antibody #60968 recognize?

    ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorigenesis and innate imm...

    Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?

    We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are: