Render Target: SSR
Render Timestamp: 2025-01-23T06:27:07.225Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
1% for the planet logo
< Back to Support Article Search Results

Does the presence of casein in the blocking solution matter for IHC experiments?

Reagents that contain casein, such as milk, may result in a reduced signal or lack of signal with some antibodies in IHC (most notably with phospho-specific antibodies). Therefore, casein in the blocking solution for IHC may be problematic. Given this, we routinely block in 1X TBST+5% Normal Goat Serum #5425 (NGS) or Animal-Free Blocking Solution (5X) #15019 when performing IHC experiments.

Last updated: September 12, 2024

Was this article helpful?