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What is the depth of sequencing reads per sample required in a CUT&RUN assay?

We suggest a sequencing depth of 3-5 million reads per sample. Because of the very low background signal generated in CUT&RUN, this sequencing depth is usually sufficient for histone modifications and transcription factors. We have found that less than 3 million does not work and greater than 15 million greatly increases the number of duplicate reads.

Last updated: September 12, 2024

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