Render Target: SSR
Render Timestamp: 2025-01-23T08:55:47.894Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
< Back to Support Article Search Results

What is the expected yield of a CUT&Tag DNA library?

The expected yield of an amplified CUT&Tag DNA library depends on the DNA quantification system used.

  • If you are using a NanoDrop Spectrophotometer or QIAxpert System, the typical reading is between 10-20 ng/µL for histone targets and 5-12 ng/µL for non-histone targets. If the library concentration is lower than 3 ng/µL with the NanoDrop or QIAxpert System, please refer to the CUT&Tag Troubleshooting Guide before sequencing your samples.
  • If you are using a Qubit Fluorometric Quantification System or PicoGreen dsDNA Assay, the typical reading is between 3-10 ng/µL for histone targets and could be lower than 1 ng/µL for non-histone targets.
However, low yield doesn't necessarily indicate a failure of the CUT&Tag experiment. We suggest continuing with next-generation sequencing (NGS) if the Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 positive control generates the expected library yield, or if the quantitative PCR (qPCR) QC of the DNA library generates a good signal-to-noise ratio. A low CUT&Tag DNA library yield shouldn't stop you from obtaining quality NGS data.

Related blog: CUT&Tag DNA Library Yield: What to Do if Yours Is Too Low to Detect with an Agilent Bioanalyzer or TapeStation System

Last updated: September 12, 2024

Was this article helpful?

Technical Support

Email: support@cellsignal.com

Send Us a Message

Call: 877-678-8324

Customer Support

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355

Fax: 866-432-6112

Contact Sales

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355