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What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?

Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.

WaLP recognizes and cleaves SUMO proteins containing AGG, SGG, TGG or VGG motifs at the SUMO c-terminus.

One should verify that the predicted SUMO sequence from their model organism contains these motifs at the c-terminus.

Last updated: September 12, 2024

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