Render Target: SSR
Render Timestamp: 2025-01-21T03:40:34.961Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
< Back to Support Article Search Results

What time and temperature do you recommend for secondary antibody incubation in my IF experiment?

We recommend a 1 hour incubation at room temperature. We have found that prolonged incubation (>2hrs) and elevated incubation temperatures (>25C) can promote non-specific binding.

Last updated: September 12, 2024

Was this article helpful?

Technical Support

Email: support@cellsignal.com

Send Us a Message

Call: 877-678-8324

Customer Support

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355

Fax: 866-432-6112

Contact Sales

Email: orders@cellsignal.com

Send Us a Message

Call: 877-616-2355