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Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: 

  • β-Actin (13E5) Rabbit mAb #4970
  • β-Actin (D6A8) Rabbit mAb #8457
  • β-Actin (8H10D10) Mouse mAb #3700
  • β-Actin (E4D9Z) Mouse mAb #58169.
All of these antibodies work well and there is no preference for this application. Please note that all require methanol permeabilization, therefore, optimization may be necessary for multiplexing. 

Last updated: September 12, 2024

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