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Why do the VASP antibodies detect a doublet?

The 48 kDa band represents VASP phosphorylated at Ser239 and the 50 kDa band represents VASP phosphorylated at both Ser239 and Ser157. The hyper-phosphorylation that occurs when both serines are phosphorylated leads to a shift in the molecular weight. If you would like more information, please refer to Aizawa et al. (2003) Circ Res 93, 406-13 (PMID: 12919948; https://www.ncbi.nlm.nih.gov/pubmed/12919948).

Last updated: September 12, 2024

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