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Why is a doublet observed by western blot with your ZEB2 antibody #97885?

ZEB2 mRNA is alternatively spliced and there are post-translational modifications (PTMs), such as ubiquitination and sumoylation, that may be the cause of the larger, 210kDa band. The potential modification sites on the ZEB2 protein can be found at https://www.phosphosite.org/proteinAction.action?id=15497&showAllSites=true. Please also see the UniProt page for human ZEB2 for more sequence and protein information (UniProt: O60315, https://www.uniprot.org/uniprot/O60315).

Last updated: September 12, 2024

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