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Render Timestamp: 2025-01-21T16:29:39.048Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-05-10 22:31:21.168
Product last modified at: 2025-01-16T19:00:08.547Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

COX IV (4D11-B3-E8) Mouse mAb #11967

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: COX IV (4D11-B3-E8) Mouse mAb
Western blot analysis of extracts from various cell lines using COX IV (4D11-B3-E8) Mouse mAb.

To Purchase # 11967

Cat. #

Size

11967T
20 µl
11967S
100 µl

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 17
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:50
Immunofluorescence (Immunocytochemistry) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #85707.

Protocol

Specificity / Sensitivity

COX IV (4D11-B3-E8) Mouse mAb recognizes endogenous levels of total COX IV protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COX IV protein.

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
For Research Use Only. Not For Use In Diagnostic Procedures.
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