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Render Timestamp: 2025-01-22T02:08:36.790Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:31:12.393
Product last modified at: 2025-01-01T09:06:31.654Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-BRIP1/FANCJ (Thr1133) Antibody #11983

Filter:
  • WB
  • IP
Western Blotting Image 1: Phospho-BRIP1/FANCJ (Thr1133) Antibody
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-BRIP1/FANCJ (Thr1133) Antibody (upper) or BRIP1/FANCJ Antibody #4578 (lower).

To Purchase # 11983

Cat. #

Size

11983S
100 µl

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 145
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-BRIP1/FANCJ (Thr1133) Antibody recognizes endogenous levels of BRIP1/FANCJ protein only when phosphorylated at Thr1133. This antibody also cross-reacts with a protein of unknown origin at ~130 kDa.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr1133 of human BRIP1/FANCJ protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of BACH1 mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor (6).
Phosphorylation of BACH1 at Thr1133 is thought to be involved in regulation of the replication checkpoint and is required for the interaction of BACH1 with TopBP1 (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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