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Render Timestamp: 2025-01-21T04:31:10.676Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-09 15:01:08.271
Product last modified at: 2025-01-06T15:00:09.821Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SGK1 (D27C11) Rabbit mAb #12103

Filter:
  • WB
  • IP
Western Blotting Image 1: SGK1 (D27C11) Rabbit mAb
Western blot analysis of extracts from SK-MEL-28, SK-MEL-5, and HSG cells using SGK1 (D27C11) Rabbit mAb.

To Purchase # 12103

Cat. #

Size

12103T
20 µl
12103S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45-60
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SGK1 (D27C11) Rabbit mAb recognizes endogenous levels of total SGK1 protein. This antibody does not cross-react with SGK2 or SGK3.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human SGK1 protein.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).
In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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