Ki-67 (D3B5) Rabbit mAb (IHC Formulated) #12202

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IHC-Immunohistochemistry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Immunohistochemistry (Paraffin)	1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #34330.

Protocol

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (IHC Formulated) recognizes endogenous levels of murine Ki-67 protein. It will also detect endogenous levels of human Ki-67 protein; however, Ki-67 (D2H10) Rabbit mAb #9027 is recommended for the detection of human Ki-67 protein in paraffin-embedded tissues.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of Ki-67 protein.

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

Database Links

UniProt ID: P46013

Entrez-Gene Id: 4288

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For Research Use Only. Not For Use In Diagnostic Procedures.

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