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Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:25:26.802
Product last modified at: 2025-01-07T21:30:09.469Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Pumilio 1 Antibody #12322

Filter:
  • WB
  • IP
Western Blotting Image 1: Pumilio 1 Antibody
Western blot analysis of extracts from various cell lines using Pumilio 1 Antibody.

To Purchase # 12322

Cat. #

Size

12322S
100 µl

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 140
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Pumilio 1 Antibody recognizes endogenous levels of total pumilio 1 protein.

Species Reactivity:

Human, Mouse

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human pumilio 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Pumilio 1 and Pumilio 2 (PUM1, PUM2, or Pumilio homolog 1 and 2, respectively) are evolutionarily conserved RNA binding proteins that are thought to repress translation and stability of mRNA targets by binding to the 3'-UTR of specific RNA sequences (1). Pumilio proteins have been implicated in the regulation of genes involved in embryogenesis and germline cell development (2). Research studies have shown that PUM2 may have a role in neural stem cell fate decisions (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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