Immunofluorescence Application Solutions Kit #12727
Product Information
Reagents Not Included
1. Unconjugated or conjugated primary antibody. 2. Fluorochrome-conjugated secondary antibody (if applicable). NOTE: When using any primary or fluorochrome-conjugated secondary antibody for the first time, titrate the antibody to determine which dilution allows for the strongest specific signal with the least background for your sample. 3. Methanol (if necessary). 4. Antifade mounting reagent, such as ProLong® Gold Antifade Reagent (#9071), with DAPI (#8961).
Storage
Upon receipt, #12528 and #12606 should be stored at room temperature. #12411 and #12378 should be stored at 4°C.
Protocol
Product Description
IMPORTANT: Please refer to the antibody data sheet to determine if it is validated and approved for use on cultured cell lines (IF-IC) or frozen tissues sections (IF-F) and for information regarding appropriate antibody dilution.
Some primary antibodies may require methanol fixation or permeabilzation, which will be noted on the data sheet. Methanol is not included in this kit.
Limited Uses
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