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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb #12793

Filter:
  • WB
  • IHC
  • IF
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human glutamate dehydrogenase 1 (hGluD1; +) or glutamate dehydrogenase 2 (hGluD2; +), using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

To Purchase # 12793

Cat. # Size Qty. Price
12793T 20 µl
$153
12793S 100 µl
$339

Product Specifications

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 52
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:800 - 1:3200
Immunofluorescence (Immunocytochemistry) 1:600 - 1:1200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #80063.

Protocol

Specificity / Sensitivity

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein.

Background

Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The GLUD1 gene is ubiquitously expressed (2), while the GLUD2 gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5).
For Research Use Only. Not for Use in Diagnostic Procedures.
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