Epothilone B #12986

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Product Information

Product Usage Information

Epothilone B is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 µg in 197 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	507.68 g/mol
Purity	>99%
Molecular Formula	C₂₇H₄₁NO₆S
CAS	152044-54-7
Solubility	Soluble in DMSO and EtOH at 40mg/ml.

Background

Epothilone A and B are taxol-like macrolides originally identified as antifungal, cytotoxic metabolites derived from the myxobacterium Sorangium cellulosum. Research studies demonstrate that epothilone B polymerizes tubulin into microtubules in vitro, which induces mitotic arrest at the G2/M phase and results in inhibition of cell proliferation and cytotoxicity (1-3). Cell cycle arrest at nanomolar IC₅₀ values have been observed in many cell types, including HeLa (IC₅₀ = 32 nM), Hs578T (IC₅₀ = 3 nM) (3), as well as the multiple myeloma cell lines U266 and RPMI 8226 (IC₅₀ = ~1-10 nM) (4). Investigations have shown that both epothilone A and B competitively inhibit binding of taxol to microtubules in vitro (3).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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