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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Drosophila ICE (drICE) Antibody #13085

Filter:
  • WB
Western Blotting Image 1: Drosophila ICE (drICE) Antibody
Western blot analysis of extracts from Drosophila S2 cells, untreated (-) or treated with either Cycloheximide #2112 (10 μM, 5 hr; +) or Actinomycin D (0.7 μM, 5 hr; +), using Drosophila ICE (drICE) Antibody (upper) and Cleaved Drosophila ICE (drICE) (Asp230) Antibody #9478 (lower).

To Purchase # 13085

Cat. # Size Qty. Price Ships
13085S 100 µl
$306

Supporting Data

REACTIVITY Dm
SENSITIVITY Endogenous
MW (kDa) 38, 21
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Dm-D. melanogaster 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Drosophila ICE (drICE) Antibody recognizes endogenous levels of total drICE protein, including both full-length and cleaved protein products.

Species Reactivity:

D. melanogaster

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of Drosophila ICE (drICE) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases and four encode effector caspases (reviewed in 2). drICE is a cysteine protease that cleaves baculovirus p35 and lamin DmO in vitro and acts downstream of rpr (3). drICE is proteolytically processed during apoptosis into active p21 and p12 subunits. Comparison of the in vivo activity between drICE and Dcp-1 has shown that drICE is a more effective inducer of apoptosis than Dcp-1, which plays a role in determining the rate of cell death (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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