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Render Timestamp: 2025-01-22T01:57:48.642Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:53:48.713
Product last modified at: 2025-01-08T21:30:08.920Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Securin (D2B6O) Rabbit mAb #13445

Filter:
  • WB
  • IP
Western Blotting Image 1: Securin (D2B6O) Rabbit mAb
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Securin (D2B6O) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 13445

Cat. #

Size

13445T
20 µl
13445S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 25
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Securin (D2B6O) Rabbit mAb recognizes endogenous levels of total securin protein. Based on western blot of overexpressed proteins, this antibody will also recognize securin 2 (PTTG2) to a lesser extent.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val19 of human securin (PTTG1) protein.

Background

Securin is a regulatory protein that contributes to mitotic checkpoint control, p53 activity, DNA repair, and cell migration. The securin protein prevents premature separation of sister chromatids by inhibiting the protease separase, which cleaves the cohesin complex at the onset of anaphase to allow for sister chromatid segregation (1,2). In mouse oocytes, securin regulates cyclin B stability and G2/M transition (3). Securin negatively affects the transcriptional activity of p53, preventing p53-regulated apoptosis (4). Research studies indicate that securin plays a role in microtubule nucleation, cell polarization, and cell migration (5). Additional research indicates that securin expression may be useful as a biomarker for human breast cancer (6-8). The pituitary tumor-transforming gene 1 (PTTG1) encodes for securin protein, while the highly homologous securin 2 protein is transcribed from the related PTTG2 gene. Securin 2 (PTTG2) protein lacks the ability to inhibit separase activity and may function primarily in cell adhesion and migration (9,10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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