NCAPD3 (D3H6L) Rabbit mAb #13473
- WB
- IP
To Purchase # 13473
Cat. #
Size
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 170 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Each condensin complex exhibits different localization patterns during the cell cycle and provides for distinct functions during mitosis (3-5). Condensin I is cytoplasmic during interphase and binds chromatin following the breakdown of the nuclear envelope at the end of prophase. Condensin I is required for complete dissociation of cohesin from chromosome arms, for chromosome shortening, and for normal timing of progression through pro-metaphase and metaphase. Mutations in corresponding condensin I genes result in cytokinesis defects due to the persistence of anaphase fibers. Condensin II is nuclear during interphase, but does not bind to chromatin until early prophase where it remains bound until the end of telophase. Condensin II is required for initial chromatin condensation during early prophase. Mutations in corresponding condensin II genes produce high numbers of anaphase bridges resulting from incomplete chromosome segregation. Condensin II complex subunit D3 (NCAPD3) plays a pivotal role in the loading of condensin II onto chromatin and the regulation of chromatin condensation (6,7). NCAPD3 protein contains HEAT repeat clusters that bind to mono-methyl histone H4 Lys20, a histone mark prevalent during mitosis and important for DNA repair and chromatin condensation (6). Increased mono-methyl histone H4 Lys20 levels caused by dissociation of the histone demethylase PHF8 from chromatin and increased expression of the methyltransferase SET8, leads to increased binding of NCAPD3 and condensin II to chromosomes early in mitosis (6). Phosphorylation of NCAPD3 at Thr1415 by CDK1 kinase (cdc2) leads to the recruitment of PLK1 kinase, which hyperphosphorylates condensin II and facilitates mitotic chromosome assembly (7).
- Losada, A. and Hirano, T. (2005) Genes Dev 19, 1269-87.
- Hudson, D.F. et al. (2009) Chromosome Res 17, 131-44.
- Hirota, T. et al. (2004) J Cell Sci 117, 6435-45.
- Ono, T. et al. (2004) Mol Biol Cell 15, 3296-308.
- Green, L.C. et al. (2012) J Cell Sci 125, 1591-604.
- Liu, W. et al. (2010) Nature 466, 508-12.
- Abe, S. et al. (2011) Genes Dev 25, 863-74.
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