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Render Timestamp: 2025-01-22T01:58:00.704Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:15:00.676
Product last modified at: 2025-01-01T09:05:27.337Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Spartin Antibody #13520

Filter:
  • WB
Western Blotting Image 1: Spartin Antibody
Western blot analysis of extracts from various cell lines using Spartin Antibody.

To Purchase # 13520

Cat. #

Size

13520S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 78
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Spartin Antibody recognizes endogenous levels of total spartin protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human spartin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Spastic paraplegia 20 (spartin) is encoded by the SPG20 gene in humans, which is altered in some individuals with an autosomal recessive form of hereditary spastic paraplegia known as Troyer syndrome (1,2). While Troyer syndrome research studies have yet to clearly describe the subcellular localization or function of spartin, additional work implicates spartin in endosomal trafficking, microtubule dynamics, and lipid homoeostasis (3-5). This multifunctional protein is ubiquitously expressed within the nervous system and in non-neuronal tissues, and displays a diverse pattern of cellular localization (6). The SPG20 gene promoter is hypermethylated in many cases of colorectal cancer, which results in decreased spartin expression and cytokinesis arrest. This suggests that spartin expression and methylation state could be a promising biomarker for colorectal tumors (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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