Y-27632 #13624

Name: Y-27632
Price: 135.00 USD
Availability: InStock

Product Information

Product Usage Information

Y-27632 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 312.2 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 15 min-4 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	320.3 g/mol
Purity	>98%
Molecular Formula	C₁₄H₂₁N₃O · 2HCl
CAS	129830-38-2
Solubility	Soluble in DMSO at 20mg/ml and H2O at 14mg/ml.

Background

Y-27632 is a potent and selective ATP-competitive inhibitor of Rho-associated kinases (ROCK), with K_i values of 0.22 µM and 0.30 µM for ROCK1 and ROCK2, respectively. In vitro kinase assays demonstrate that Y-27632 exhibits a 20-fold greater preference for ROCK when compared to citron kinase and protein kinase N (1). Research studies show that Y-27632 treatment of cells leads to inhibition of ROCK phosphorylation of myosin phosphatase subunit 1 (MYPT1) at both Thr853 and Thr696, with a much greater inhibition of Thr853 phosphorylation (2,3). Y-27632 selectively inhibits smooth-muscle contraction by inhibiting Ca²⁺ sensitization (4). Additional research indicates that Y-27632 inhibits dissociation-induced apoptosis of cultured human embryonic stem cells over numerous passages and increases cloning efficiency (5).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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