Render Target: STATIC
Render Timestamp: 2024-12-30T11:20:15.687Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:57.390
Product last modified at: 2024-12-17T18:48:22.510Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DNA Polymerase η (E1I7T) Rabbit mAb #13848

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DNA Polymerase η (E1I7T) Rabbit mAb recognizes endogenous levels of total POLH protein. The antibody recognizes a 40 kDa background band of unknown origin. In some cell lines, the antibody recognizes a 60 kDa band of unknown origin. This band does not correspond to the expected size of truncated POLH in GM03617 cells.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro309 of human POLH protein.

    Background

    A total of fifteen mammalian DNA polymerase enzymes catalyze the synthesis of nascent DNA during DNA replication and repair (1). DNA polymerase eta (POL η, POLH, Rad30) is one of a specialized type of DNA polymerases that function in DNA repair and translesion synthesis (TLS). POLH can accommodate and read through bulky DNA lesions such as pyrimidine dimers, which allows for continued DNA synthesis past lesions and limited stalling of replication forks (2,3). Damage inducing conditions, such as exposure to UV light or cisplatin, recruit POLH to sites of bulky DNA lesions where the polymerase interacts with PCNA (4,5). Mutations in the human POLH gene can result in a form of xeroderma pigmentosum (XPV), an autosomal recessive disorder characterized by hypersensitivity to light and susceptibility to skin cancer (6).
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