Render Target: STATIC
Render Timestamp: 2025-01-24T10:45:59.710Z
Commit: 8d9f38232df81570bbc23eaa560b31cb39dd8776
XML generation date: 2024-09-30 01:54:06.430
Product last modified at: 2025-01-01T09:06:17.926Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb #14371

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb recognizes endogenous levels of proteins that are phosphorylated at threonine within the context of a TPXK motif. The antibody does not cross-react with non-phosphorylated proteins or phospho-threonine in a different context.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-TPXK motif.

    Background

    Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transition through their association and subsequent phosphorylation of substrate proteins in a proline-directed fashion. CDKs phosphorylate a serine or threonine residue within the (S/T)PX(R/K) consensus sequence (1-5). Cell Signaling Technology has developed antibodies to phosphorylated proline-rich motifs for broad-based discovery applications. The TPXK motif is targeted for phosphorylation by CDKs 1, 2, 4, 5, and 6 (1-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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