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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Eg5 (E1L3W) Rabbit mAb #14404

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: Eg5 (E1L3W) Rabbit mAb
Western blot analysis of extracts from A-431, MCF7, and COLO 205 cells using Eg5 (E1L3W) Rabbit mAb.

To Purchase # 14404

Cat. # Size Qty. Price Ships
14404T 20 µl
$153 Jan 22
14404S 100 µl
$339 Jan 22

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 120
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunofluorescence (Immunocytochemistry) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Eg5 Rabbit mAb recognizes endogenous levels of total Eg5 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Eg5 protein.

Background

Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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