Render Target: STATIC
Render Timestamp: 2025-01-21T21:25:33.667Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:54:14.298
Product last modified at: 2025-01-01T09:06:16.513Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dinitrophenol (D1D6) Rabbit mAb #14681

Filter:
  • WB
Western Blotting Image 1: Dinitrophenol (D1D6) Rabbit mAb
Western blot analysis of dinitrophenol-conjugated IgG (IgG/DNP, 200 ng; +) and dinitrophenol-conjugated BSA (BSA/DNP, 0.001 ng; +) using Dinitrophenol (D1D6) Rabbit mAb.

To Purchase # 14681

Cat. # Size Qty. Price Ships
14681T 20 µl
$153
14681S 100 µl
$339

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • All-All Species Expected 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:10000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Dinitrophenol (D1D6) Rabbit mAb recognizes dinitrophenol-conjugated molecules.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.

Background

The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.