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Render Timestamp: 2025-01-20T13:16:53.824Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:54:15.988
Product last modified at: 2025-01-01T09:06:17.444Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PTP-PEST (D4W7W) Rabbit mAb #14735

Filter:
  • WB
  • IP
Western Blotting Image 1: PTP-PEST (D4W7W) Rabbit mAb
Western blot analysis of extracts from various cell lines using PTP-PEST (D4W7W) Rabbit mAb.

To Purchase # 14735

Cat. #

Size

14735T
20 µl
14735S
100 µl

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 110 to 125
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PTP-PEST (D4W7W) Rabbit mAb recognizes endogenous levels of total PTP-PEST protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg747 of human PTP-PEST protein.

Background

PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration, and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASP, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak, and WASP, PTP-PEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substrates such as paxillin, p130 Cas, Crk, and PSTPIP1 (8).
PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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